Compared to a typical hemacytometer with a fixed volume of 0.1 µl, the minimum measurable cell concentration of 5 × 10 3 cells/ml on the multi-volume hemacytometer is twenty times lower. A multi-volume hemacytometer can measure cell concentration with a maximum of 10 6 cells/ml to a minimum of 5 × 10 3 cells/ml. We have developed a new hemacytometer that has a multi-volume chamber with 4 different depths containing different volumes (0.1, 0.2, 0.4, 0.8 µl respectively). If the cell concentration of the unknown sample is outside the range of the hemacytometer, the sample must be prepared again by increasing or decreasing the cell concentration. The principle of both manual and automated hemacytometers is to calculate cell numbers with a fixed volume within a set measurement range (10 5 ~ 10 6 cells/ml). A hemacytometer is the standard device used to measure cell numbers in most laboratories which are typically automated to increase throughput. Cell counting has become an essential method for monitoring the viability and proliferation of cells.
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